![]() In addition, by selection of suitable cell lines, the number of viruses indexed has increased dramatically. With the utilization of cell culture technique, use of laboratory animals in experiments has decreased significantly. Typically, cell cultures are developed from tissue samples and then disaggregated by mechanical, chemical, and enzymatic methods to extract cells suitable for isolation of viruses. Introduction In the 1900s, embryonated eggs and laboratory animals were used for isolation of viruses.
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